Description
Aviva Systems Biology MYH7 ELISA Kit (Rat) (OKEH03043) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-rat Myh7 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Myh7 and incubated. The Myh7 found in the sample or standards competes with the biotinylated Myh7 for limited binding sites on the immobilized anti-rat Myh7 antibody. Excess unbound biotinylated Myh7 and sample or standard Myh7 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Myh7 captured in the well and inversely proportional to the amount of Myh7 which was contained in the sample or standard